Ethidium Bromide

Effect of ethidium bromide Ethidium bromide is a fluorescent dye and it intercalates between nucleic acids bases and provides opportunity to easily detect nucleic acid fragments in gels (Sharp et al. 1973; Boffey, 1984; Lodge et al. 2007; Harrington, 1993; Lane et al., 1992).The gel subsequently is being illuminated with an ultraviolet lamp usually by placing it on a light box. An apparatus integrated with the illumination system is used to take images of the gel with the presence of UV illumination. The gel can be subsequently photographed usually with a digital camera and images are usually shown in black and white, despite the fact that the stained nucleic acid fluoresces reddish-orange.