Jurkat T cells were infected for 7–10 days with HIV-1 expressing GFP, mixed with adherent monocyte-derived macrophages, and immediately imaged. Time-lapse imaging occurred over approximately 2 hr.
Check out the paper at (14)00388-6
Baxter, A.E.,
Rebecca A. Russell, R.A., Duncan, ., Moore, M.D., Willberg, C.B., Pablos, J.L., Finzi, A., Kaufmann, D.E., Ochsenbauer, C., Kappes, J.C., Groot, F., and Sattentau, Q.J. (2014). Macrophage Infection via Selective Capture of HIV-1-Infected CD4 T Cells. Cell Host Microbe. 16.
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