Easy CRISPR/Cas9 gRNA plasmid cloning
CRISPR/Cas9 plasmid construction can be so easy and does not require numerous steps and gel purification to get 100% efficiency. Oligo annealing cloning uses the GoldenGate method of cloning. Print protocol can be found here:
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Easy CRISPR/Cas9 gRNA plasmid cloning
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