Which sequencies can serve as recognition site for a restriction enzyme?

A palindromic sequence is a nucleic acid sequence (DNA or RNA) that is the same whether read 5’ (five-prime) to 3’ (three prime) on one strand or 5’ to 3’ on the complementary strand with which it forms a double helix. The meaning of palindrome in the context of genetics is slightly different from the definition used for words and sentences. Since a double helix is formed by two paired strands of nucleotides that run in opposite directions in the 5’-to-3’ sense, and the nucleotides always pair in the same way (Adenine (A) with Thymine (T) for DNA, with Uracil (U) for RNA; Cytosine (C) with Guanine (G)), a (single-stranded) nucleotide sequence is said to be a palindrome if it is equal to its reverse complement. For example, the DNA sequence ACCTAGGT is palindromic because its nucleotide-by-nucleotide complement is TGGATCCA, and reversing the order of the nucleotides in the complement gives the original sequence. A palindromic nucleotide sequence can form a hairpin. Palindromic DNA motifs are found in most genomes or sets of genetic instructions. Palindromic motifs are made by the order of the nucleotides that specify the complex chemicals (proteins) which, as a result of those genetic instructions, the cell is to produce. They have been specially researched in bacterial chromosomes and in the so-called Bacterial Interspersed Mosaic Elements (BIMEs) scattered over them. Recently a research genome sequencing project discovered that many of the bases on the Y chromosome are arranged as palindromes.A palindrome structure allows the Y chromosome to repair itself by bending over at the middle if one side is damaged. Palindromes also appear to be found frequently in proteins, but their role in the protein function is not clearly known. It is recently suggested that the prevalence existence of palindromes in peptides might be related to the prevalence of low-complexity regions in proteins, as palindromes are frequently associated with low-complexity sequences. Their prevalence might be also related to an alpha helical formation propensity of these sequences, or in formation of protein/protein complexes. Restriction Enzyme Sites Palindromic sequences play an important role in molecular biology. Because a DNA sequence is double stranded, the base pairs are read, not just the bases on one strand, to determine a palindrome. Many restriction endonucleases (restriction enzymes) recognize specific palindromic sequences and cut them. The restriction enzyme EcoR1 recognizes the following palindromic sequence: 5’- G A A T T C -3’ 3’- C T T A A G -5’ #complementaryStrand #GeneticExamQuestionsSolutions #Genetics101 #restrictionEndonucleases #palindromicSequence #NucleicAcidSequence #Allele #GeneticsExamQuestionsSolutions #DNAMotifs #MolecularBiology #Proteins #alleles #Genetics #palindromicNucleotideSequence #RestrictionEnzymeSites #GeneticsLecture #DNA #agarose #DNABands #usingAGelElectrophoresisMachine #DNAFingerprinting #howDoesAGelElectrophoresisMachineWork #animation #tutorial #biology #science