How to Prepare Agar Stock Solution - Laboratory Calculations

Cell cloning is commonly performed in a Jello-like semi-solid support structure made with a solution of a substance called agar, which is allowed to set, just like Jello. This support structure also contains a nutrient solution to provide nourishment for the cells. While the agar solution needs to be sterilized by heating to a high temperature (autoclaving) to render it sterile, the nutrients in the nutrient solution will be destroyed upon autoclaving. Therefore, the agar solution is commonly prepared at a higher concentration in water (agar stock solution), and mixed with a nutrient solution that contains the nutrients at a higher concentration than needed (nutrient stock solution). When the two solutions are mixed together and diluted with sterile water, the agar and the nutrients are both diluted sufficiently to obtain the right concentrations of each. #gellMedia #agaroseGell #autoclaving #Agar #laboratoryCalculation #dilutions